Details

Autor(en) / Beteiligte

• Chitraju, Chandramohan
• Fischer, Alexander W.
• Farese, Robert V.
• Walther, Tobias C.

Titel

Lipid Droplets in Brown Adipose Tissue Are Dispensable for Cold-Induced Thermogenesis

Ist Teil von

• Cell reports (Cambridge), 2020-11, Vol.33 (5), p.108348-108348, Article 108348

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2020

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Brown adipocytes store metabolic energy as triglycerides (TGs) in lipid droplets (LDs). Fatty acids released from brown adipocyte LDs by lipolysis are thought to activate and fuel UCP1-mediated thermogenesis. Here, we test this hypothesis by preventing fatty acid storage in murine brown adipocytes through brown adipose tissue (BAT)-specific deletions of the TG synthesis enzymes DGAT1 and DGAT2 (BA-DGAT KO). Despite the absence of TGs in brown adipocytes, BAT is functional, and BA-DGAT-KO mice maintain euthermia during acute or chronic cold exposure. As apparent adaptations to the lack of TG, brown adipocytes of BA-DGAT-KO mice appear to use circulating glucose and fatty acids, and stored glycogen, to fuel thermogenesis. Moreover, BA-DGAT-KO mice are resistant to diet-induced glucose intolerance, likely because of increased glucose disposal by BAT. We conclude that TGs in BAT are dispensable for its contribution to cold-induced thermogenesis, at least when other fuel sources are available. [Display omitted] •Lipid droplets are absent in brown adipocytes of mice lacking both DGAT1 and DGAT2•Mice lacking lipid droplets in brown adipocytes maintain euthermia during cold exposure•Brown adipocytes lacking lipid droplets use glucose, FAs, and glycogen as fuels•Mice lacking triglycerides in brown adipocytes are resistant to glucose intolerance Chitraju et al. show that mice lacking triglycerides and lipid droplets in brown adipocytes maintain euthermia during acute or chronic cold exposure by using circulating glucose and fatty acids and stored glycogen to fuel thermogenesis.