Details

Autor(en) / Beteiligte

• Okuda, Aya
• Inoue, Rintaro
• Morishima, Ken
• Saio, Tomohide
• Yunoki, Yasuhiro
• Yagi-Utsumi, Maho
• Yagi, Hirokazu
• Shimizu, Masahiro
• Sato, Nobuhiro
• Urade, Reiko
• Kato, Koichi
• Sugiyama, Masaaki

Titel

Deuteration Aiming for Neutron Scattering

Ist Teil von

• Biophysics and Physicobiology, 2021, Vol.18, pp.16-27

Ort / Verlag

Japan: The Biophysical Society of Japan

Erscheinungsjahr

2021

Link zum Volltext

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• The distinguished feature of neutron as a scattering probe is an isotope effect, especially the large difference in neutron scattering length between hydrogen and deuterium. The difference renders the different visibility between hydrogenated and deuterated proteins. Therefore, the combination of deuterated protein and neutron scattering enables the selective visualization of a target domain in the complex or a target protein in the multi-component system. Despite of this fascinating character, there exist several problems for the general use of this method: difficulty and high cost for protein deuteration, and control and determination of deuteration ratio of the sample. To resolve them, the protocol of protein deuteration techniques is presented in this report. It is strongly expected that this protocol will offer more opportunity for conducting the neutron scattering studies with deuterated proteins.