Nature communications, 2019-02, Vol.10 (1), p.650-650, Article 650
2019
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- Autor(en) / Beteiligte
- Titel
- Single-cell analysis reveals fibroblast heterogeneity and myeloid-derived adipocyte progenitors in murine skin wounds
- Ist Teil von
- Nature communications, 2019-02, Vol.10 (1), p.650-650, Article 650
- Ort / Verlag
- England: Nature Publishing Group
- Erscheinungsjahr
- 2019
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- During wound healing in adult mouse skin, hair follicles and then adipocytes regenerate. Adipocytes regenerate from myofibroblasts, a specialized contractile wound fibroblast. Here we study wound fibroblast diversity using single-cell RNA-sequencing. On analysis, wound fibroblasts group into twelve clusters. Pseudotime and RNA velocity analyses reveal that some clusters likely represent consecutive differentiation states toward a contractile phenotype, while others appear to represent distinct fibroblast lineages. One subset of fibroblasts expresses hematopoietic markers, suggesting their myeloid origin. We validate this finding using single-cell western blot and single-cell RNA-sequencing on genetically labeled myofibroblasts. Using bone marrow transplantation and Cre recombinase-based lineage tracing experiments, we rule out cell fusion events and confirm that hematopoietic lineage cells give rise to a subset of myofibroblasts and rare regenerated adipocytes. In conclusion, our study reveals that wounding induces a high degree of heterogeneity among fibroblasts and recruits highly plastic myeloid cells that contribute to adipocyte regeneration.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2041-1723eISSN: 2041-1723DOI: 10.1038/s41467-018-08247-xTitel-ID: cdi_doaj_primary_oai_doaj_org_article_099a0aa5ccec4a9394fa9efe30aa4c89
- Format
- –
- Schlagworte
- Adipocytes, Adipocytes - cytology, Adipocytes - metabolism, Animals, Blotting, Western, Bone marrow, Bone marrow transplantation, Cell fusion, Cells, Cultured, Clusters, Contractility, Cre recombinase, Female, Fibroblasts, Fibroblasts - cytology, Fibroblasts - metabolism, Follicles, Gene sequencing, Hemopoiesis, Heterogeneity, Male, Mice, Myeloid cells, Phenotypes, Regeneration, Ribonucleic acid, RNA, Sequence Analysis, RNA, Single-Cell Analysis - methods, Skin, Skin - cytology, Stem Cells - cytology, Stem Cells - metabolism, Transplantation, Wound healing, Wound Healing - physiology