Details

Autor(en) / Beteiligte

• Reshef, Ronen
• Kudryavitskaya, Elena
• Shani-Narkiss, Haran
• Isaacson, Batya
• Rimmerman, Neta
• Mizrahi, Adi
• Yirmiya, Raz

Titel

The role of microglia and their CX3CR1 signaling in adult neurogenesis in the olfactory bulb

Ist Teil von

• eLife, 2017-12, Vol.6

Ort / Verlag

England: eLife Science Publications, Ltd

Erscheinungsjahr

2017

Quelle

EZB Free E-Journals

Beschreibungen/Notizen

• Microglia play important roles in perinatal neuro- and synapto-genesis. To test the role of microglia in these processes during adulthood, we examined the effects of microglia depletion, via treatment of mice with the CSF-1 receptor antagonist PLX5622, and abrogated neuronal-microglial communication in CX3C receptor-1 deficient ( ) mice. Microglia depletion significantly lowered spine density in young (developing) but not mature adult-born-granule-cells (abGCs) in the olfactory bulb. Two-photon time-lapse imaging indicated that microglia depletion reduced spine formation and elimination. Functionally, odor-evoked responses of mitral cells, which are normally inhibited by abGCs, were increased in microglia-depleted mice. In mice, abGCs exhibited reduced spine density, dynamics and size, concomitantly with reduced contacts between -deficient microglia and abGCs' dendritic shafts, along with increased proportion of microglia-contacted spines. Thus, during adult neurogenesis, microglia regulate the elimination (pruning), formation, and maintenance of synapses on newborn neurons, contributing to the functional integrity of the olfactory bulb circuitry.