Details

Autor(en) / Beteiligte

• Okumura, Fumihiko
• Oki, Nodoka
• Fujiki, Yuha
• Ikuta, Rio
• Osaki, Kana
• Hamada, Shun
• Nakatsukasa, Kunio
• Hisamoto, Naoki
• Hara, Taichi
• Kamura, Takumi

Titel

ZSWIM8 is a myogenic protein that partly prevents C2C12 differentiation

Ist Teil von

• Scientific reports, 2021-10, Vol.11 (1), p.20880-20880, Article 20880

Ort / Verlag

England: Nature Publishing Group

Erscheinungsjahr

2021

Quelle

MEDLINE

Beschreibungen/Notizen

• Cell adhesion molecule-related/downregulated by oncogenes (Cdon) is a cell-surface receptor that mediates cell-cell interactions and positively regulates myogenesis. The cytoplasmic region of Cdon interacts with other proteins to form a Cdon/JLP/Bnip-2/CDC42 complex that activates p38 mitogen-activated protein kinase (MAPK) and induces myogenesis. However, Cdon complex may include other proteins during myogenesis. In this study, we found that Cullin 2-interacting protein zinc finger SWIM type containing 8 (ZSWIM8) ubiquitin ligase is induced during C2C12 differentiation and is included in the Cdon complex. We knocked-down Zswim8 in C2C12 cells to determine the effect of ZSWIM8 on differentiation. However, we detected neither ZSWIM8-dependent ubiquitination nor the degradation of Bnip2, Cdon, or JLP. In contrast, ZSWIM8 knockdown accelerated C2C12 differentiation. These results suggest that ZSWIM8 is a Cdon complex-included myogenic protein that prevents C2C12 differentiation without affecting the stability of Bnip2, Cdon, and JLP.