Details

Autor(en) / Beteiligte

• Zuber, Philipp K
• Artsimovitch, Irina
• NandyMazumdar, Monali
• Liu, Zhaokun
• Nedialkov, Yuri
• Schweimer, Kristian
• Rösch, Paul
• Knauer, Stefan H

Titel

The universally-conserved transcription factor RfaH is recruited to a hairpin structure of the non-template DNA strand

Ist Teil von

• eLife, 2018-05, Vol.7

Ort / Verlag

England: eLife Sciences Publications Ltd

Erscheinungsjahr

2018

Link zum Volltext

Quelle

EZB Free E-Journals

Beschreibungen/Notizen

• RfaH, a transcription regulator of the universally conserved NusG/Spt5 family, utilizes a unique mode of recruitment to elongating RNA polymerase to activate virulence genes. RfaH function depends critically on an sequence, an exemplar of a consensus pause, in the non-template DNA strand of the transcription bubble. We used structural and functional analyses to elucidate the role of in RfaH recruitment. Our results demonstrate that induces pausing to facilitate RfaH binding and establishes direct contacts with RfaH. Strikingly, the non-template DNA forms a hairpin in the RfaH: complex structure, flipping out a conserved T residue that is specifically recognized by RfaH. Molecular modeling and genetic evidence support the notion that hairpin is required for RfaH recruitment. We argue that both the sequence and the structure of the non-template strand are read out by transcription factors, expanding the repertoire of transcriptional regulators in all domains of life.