International journal of molecular sciences, 2020-12, Vol.22 (1), p.368
2020
Details
- Autor(en) / Beteiligte
- Titel
- The Status of EGFR Modulates the Effect of miRNA-200c on ZEB1 Expression and Cell Migration in Glioblastoma Cells
- Ist Teil von
- International journal of molecular sciences, 2020-12, Vol.22 (1), p.368
- Ort / Verlag
- Switzerland: MDPI AG
- Erscheinungsjahr
- 2020
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Migration of glioblastoma cells into surrounding tissue is one of the main features that makes this tumor incurable. We evaluated whole-genome miRNA expression profiling associated with different EGFR amplification patterns in 30 cases of primary glioblastoma. From the 64 miRNAs that showed differential expression between tumors with a high level of EGFR amplification and tumors without EGFR amplification, 40% were related with cell migration, being miR-200c the most differentially expressed between these two groups. We investigated the effect of miR-200c on ZEB1 expression and cell migration in an in vitro transfection model with a miR-200c mimic, a miR-200c inhibitor and siRNA targeting EGFR in three short-term cultures with different levels of EGFR amplification obtained from resected glioblastomas. The cell culture with the highest EGFR amplification level presented the lowest miR-200c expression and the status of EGFR modulated the effect of miR-200c on ZEB1 expression. Silencing EGFR led to miR-200c upregulation and ZEB1 downregulation in transfected cultures, except in the presence of high levels of EGFR. Likewise, miR-200c upregulation decreased ZEB1 expression and inhibited cell migration, especially when EGFR was not amplified. Our results suggest that modulating miR-200c may serve as a novel therapeutic approach for glioblastoma depending on EGFR status.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1422-0067, 1661-6596eISSN: 1422-0067DOI: 10.3390/ijms22010368Titel-ID: cdi_doaj_primary_oai_doaj_org_article_07a8cb4753bd415eaec01bf2de350851
- Format
- –
- Schlagworte
- Apoptosis, Biomarkers, Tumor - genetics, Biomarkers, Tumor - metabolism, Brain cancer, Cell adhesion & migration, Cell culture, cell migration, Cell Movement, Cell Proliferation, EGFR amplification, Epidermal growth factor receptors, ErbB Receptors - genetics, Gene Amplification, Gene expression, Gene Expression Regulation, Neoplastic, Genomes, Glioblastoma, Glioblastoma - genetics, Glioblastoma - metabolism, Glioblastoma - pathology, Glioblastoma cells, Humans, MicroRNAs, MicroRNAs - genetics, miR-200c, miRNA, Mutation, Prognosis, Roles, siRNA, Transcription factors, Transfection, Tumor Cells, Cultured, Tumors, ZEB1, Zinc Finger E-box-Binding Homeobox 1 - genetics, Zinc Finger E-box-Binding Homeobox 1 - metabolism