Details

Autor(en) / Beteiligte

• Watanabe, Yasunori
• Mendonça, Luiza
• Allen, Elizabeth R
• Howe, Andrew
• Lee, Mercede
• Allen, Joel D
• Chawla, Himanshi
• Pulido, David
• Donnellan, Francesca
• Davies, Hannah
• Ulaszewska, Marta
• Belij-Rammerstorfer, Sandra
• Morris, Susan
• Krebs, Anna-Sophia
• Dejnirattisai, Wanwisa
• Mongkolsapaya, Juthathip
• Supasa, Piyada
• Screaton, Gavin R
• Green, Catherine M
• Lambe, Teresa
• Zhang, Peijun
• Gilbert, Sarah C
• Crispin, Max

Titel

Native-like SARS-CoV-2 Spike Glycoprotein Expressed by ChAdOx1 nCoV-19/AZD1222 Vaccine

Ist Teil von

• ACS central science, 2021-04, Vol.7 (4), p.594-602

Ort / Verlag

United States: American Chemical Society

Erscheinungsjahr

2021

Link zum Volltext

Quelle

Electronic Journals Library - Freely accessible e-journals

Beschreibungen/Notizen

• Vaccine development against the SARS-CoV-2 virus focuses on the principal target of the neutralizing immune response, the spike (S) glycoprotein. Adenovirus-vectored vaccines offer an effective platform for the delivery of viral antigen, but it is important for the generation of neutralizing antibodies that they produce appropriately processed and assembled viral antigen that mimics that observed on the SARS-CoV-2 virus. Here, we describe the structure, conformation, and glycosylation of the S protein derived from the adenovirus-vectored ChAdOx1 nCoV-19/AZD1222 vaccine. We demonstrate native-like post-translational processing and assembly, and reveal the expression of S proteins on the surface of cells adopting the trimeric prefusion conformation. The data presented here confirm the use of ChAdOx1 adenovirus vectors as a leading platform technology for SARS-CoV-2 vaccines.