Details

Autor(en) / Beteiligte

• Armitano, Joshua
• Redder, Peter
• Guimarães, Vanessa A.
• Linder, Patrick

Titel

An Essential Factor for High Mg2+ Tolerance of Staphylococcus aureus

Ist Teil von

• Frontiers in microbiology, 2016-11, Vol.7, p.1888-1888

Ort / Verlag

Frontiers Media S.A

Erscheinungsjahr

2016

Quelle

Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals

Beschreibungen/Notizen

• Internal bacterial concentration of Mg 2+ , the most abundant divalent cation in living cells, is estimated to be in the single millimolar range. However, many bacteria will thrive in media with only micromolars of Mg 2+ , by using a range of intensely studied and highly efficient import mechanisms, as well as in media with very high magnesium concentration, presumably mediated by currently unknown export mechanisms. Staphylococcus aureus has a particularly high Mg 2+ tolerance for a pathogen, growing unimpaired in up to 770 mM Mg 2+ , and we here identify SA0657, a key factor in this tolerance. The predicted domain structure of SA0657 is shared with a large number of proteins in bacteria, archaea and even eukarya, for example CorB from Salmonella and the human CNNM protein family. One of the shared domains, a CBS pair potentially involved in Mg 2+ sensing, contains the conserved Glycine326 which we establish to be a key residue for SA0657 function. In light of our findings, we propose the name MpfA, Magnesium Protection Factor A, for SA0657.