Details

Autor(en) / Beteiligte

• Aziz, Fatkhanuddin
• Hisatsune, Junzo
• Ono, Hisaya K
• Kajimura, Junko
• Yu, Liansheng
• Masuda, Kanako
• Kitagawa, Hiroki
• Sato'o, Yusuke
• Yahara, Koji
• Yamaoka, Mika
• Nakane, Akio
• Kawasaki, Hiroshi
• Obata, Shoko
• Fukushima-Nomura, Ayano
• Ito, Yoshihiro
• Aung, Meiji Soe
• Amagai, Masayuki
• Salasia, Siti Isrina Oktavia
• Ohge, Hiroki
• Kusunoki, Yoichiro
• Sugai, Motoyuki
• Zarrilli, Raffaele

Titel

Genomic analysis and identification of a novel superantigen, SargEY, in Staphylococcus argenteus isolated from atopic dermatitis lesions

Ist Teil von

• mSphere, 2024-07, Vol.9 (7), p.e0050524

Ort / Verlag

United States: American Society for Microbiology

Erscheinungsjahr

2024

Quelle

MEDLINE

Beschreibungen/Notizen

• During surveillance of in lesions from patients with atopic dermatitis (AD), we isolated , a species registered in 2011 as a new member of the genus and previously considered a lineage of . Genome sequence comparisons between isolates and representative clinical isolates from various origins revealed that the genome from AD patients closely resembles that of causing skin infections. We previously reported that 17%-22% of isolated from skin infections produce staphylococcal enterotoxin Y (SEY), which predominantly induces T-cell proliferation via the T-cell receptor (TCR) Vα pathway. Complete genome sequencing of isolates revealed a gene encoding a protein similar to superantigen SEY, designated as SargEY, on its chromosome. Population structure analysis of revealed that these isolates are ST2250 lineage, which was the only lineage positive for the SEY-like gene among . Recombinant SargEY demonstrated immunological cross-reactivity with anti-SEY serum. SargEY could induce proliferation of human CD4 and CD8 T cells, as well as production of TNF-α and IFN-γ. SargEY showed emetic activity in a marmoset monkey model. S EY and SET (a phylogenetically close but uncharacterized SE) revealed their dependency on TCR Vα in inducing human T-cell proliferation. Additionally, TCR sequencing revealed other previously undescribed Vα repertoires induced by SEH. S EY and SEY may play roles in exacerbating the respective toxin-producing strains in AD. is frequently isolated from active lesions of atopic dermatitis (AD) patients. We reported that 17%-22% of isolated from AD patients produced a novel superantigen staphylococcal enterotoxin Y (SEY). Unlike many . superantigens that activate T cells via T-cell receptor (TCR) Vß, SEY activates T cells via TCR Vα and stimulates cytokine secretion. was isolated from AD patients during the surveillance for . Phylogenetic comparison of the genome indicated that the isolate was very similar to causing skin infections. The isolate encoded a SEY-like protein, designated SargEY, which, like SEY, activated T cells via the TCR Vα. ST2250 is the only lineage positive for SargEY gene. ST2250 harboring a superantigen SargEY gene may be a novel staphylococcal clone that infects human skin and is involved in the exacerbation of AD.