International journal of molecular sciences, 2017-01, Vol.18 (1), p.218-218
2017
Details
- Autor(en) / Beteiligte
- Titel
- Affinin (Spilanthol), Isolated from Heliopsis longipes, Induces Vasodilation via Activation of Gasotransmitters and Prostacyclin Signaling Pathways
- Ist Teil von
- International journal of molecular sciences, 2017-01, Vol.18 (1), p.218-218
- Ort / Verlag
- Switzerland: MDPI AG
- Erscheinungsjahr
- 2017
- Link zum Volltext
- Quelle
- Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals
- Beschreibungen/Notizen
- roots have been widely used in Mexican traditional medicine to relieve pain, mainly, toothaches. Previous studies have shown that affinin, the major alkamide of these roots, induces potent antinociceptive and anti-inflammatory activities. However, the effect of root extracts and affinin on the cardiovascular system have not been investigated so far. In the present study, we demonstrated that the dichloromethane and ethanolic extracts of roots, and affinin, isolated from these roots, produce a concentration-dependent vasodilation of rat aorta. Affinin-induced vasorelaxation was partly dependent on the presence of endothelium and was significantly blocked in the presence of inhibitors of NO, H₂S, and CO synthesis (N -nitro-l-arginine methyl ester (l-NAME), dl-propargylglycine (PAG), and chromium mesoporphyrin (CrMP), respectively); K⁺ channel blockers (glibenclamide (Gli) and tetraethyl ammonium (TEA)), and guanylate cyclase and cyclooxygenase inhibitors (1 -[1,2,4]oxadiazolo[4,3- ]quinoxalin-1-one (ODQ) and indomethacin (INDO), respectively). Our results demonstrate, for the first time, that affinin induces vasodilation by mechanisms that involve gasotransmitters, and prostacyclin signaling pathways. These findings indicate that this natural alkamide has therapeutic potential in the treatment of cardiovascular diseases.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1422-0067, 1661-6596eISSN: 1422-0067DOI: 10.3390/ijms18010218Titel-ID: cdi_doaj_primary_oai_doaj_org_article_047d3fc50b09489298553d935db227f9
- Format
- –
- Schlagworte
- affinin, Amides - chemistry, Amides - isolation & purification, Amides - pharmacology, Ammonium, Animals, Aorta, Aorta - drug effects, Aorta - physiology, Arginine, Asteraceae - chemistry, Cardiovascular diseases, Cardiovascular system, Chromium, Cyclic AMP - metabolism, Dichloromethane, Endothelium, Endothelium, Vascular - drug effects, Endothelium, Vascular - physiology, Epoprostenol - metabolism, gasotransmitters, Gasotransmitters - metabolism, Glibenclamide, Guanylate cyclase, Heliopsis, Heliopsis longipes, Hydrogen sulfide, Indomethacin, Indomethacin - pharmacology, Inflammation, Inhibitors, Male, Methylene Chloride, Models, Biological, NG-Nitroarginine methyl ester, Nitric oxide, Pain, Pain perception, Phytochemicals - pharmacology, Plant Extracts - pharmacology, Plant Roots - chemistry, Polyunsaturated Alkamides, Potassium channels, Potassium Channels - metabolism, Prostacyclin, Prostaglandin endoperoxide synthase, rat aorta, Rats, Wistar, Rodents, Signal transduction, Signal Transduction - drug effects, Vasodilation, Vasodilation - drug effects