Neurobiology of disease, 2009-06, Vol.34 (3), p.487-500
2009
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- Proteomic identification of dopamine-conjugated proteins from isolated rat brain mitochondria and SH-SY5Y cells
- Ist Teil von
- Neurobiology of disease, 2009-06, Vol.34 (3), p.487-500
- Ort / Verlag
- United States: Elsevier Inc
- Erscheinungsjahr
- 2009
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Abstract Dopamine oxidation has been previously demonstrated to cause dysfunction in mitochondrial respiration and membrane permeability, possibly related to covalent modification of critical proteins by the reactive dopamine quinone. However, specific mitochondrial protein targets have not been identified. In this study, we utilized proteomic techniques to identify proteins directly conjugated with14 C-dopamine from isolated rat brain mitochondria exposed to radiolabeled dopamine quinone (150 μM) and differentiated SH-SY5Y cells treated with14 C-dopamine (150 μM). We observed a subset of rat brain mitochondrial proteins that were covalently modified by14 C-dopamine, including chaperonin, ubiquinol-cytochrome c reductase core protein 1, glucose regulated protein 75/mitochondrial HSP70/mortalin, mitofilin, and mitochondrial creatine kinase. We also found the Parkinson's disease associated proteins ubiquitin carboxy-terminal hydrolase L1 and DJ-1 to be covalently modified by dopamine in both brain mitochondrial preparations and SH-SY5Y cells. The susceptibility of the identified proteins to covalent modification by dopamine may carry implications for their role in the vulnerability of dopaminergic neurons in Parkinson's disease pathogenesis.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0969-9961eISSN: 1095-953XDOI: 10.1016/j.nbd.2009.03.004Titel-ID: cdi_doaj_primary_oai_doaj_org_article_0344410d5e974f18b8d2ce813b3045f2
- Format
- –
- Schlagworte
- Animals, Benzoquinones - pharmacology, Brain - drug effects, Brain - physiology, Carbon Radioisotopes - pharmacology, Cell Line, Tumor, Chaperonins - metabolism, Creatine Kinase, Mitochondrial Form - metabolism, DJ-1, Dopamine - metabolism, Dopamine - pharmacology, Dopamine oxidation, Electron Transport Complex III - metabolism, HSP70 Heat-Shock Proteins - metabolism, Humans, Microtubule-Associated Proteins - metabolism, Mitochondria - drug effects, Mitochondria - physiology, Mitochondrial creatine kinase, Mitochondrial Proteins - metabolism, Mitochondrion, Mitofilin, Neurology, Oxidants - pharmacology, Oxidation-Reduction - drug effects, Parkinson's disease, Protein Deglycase DJ-1, Protein modification, Proteomics, Rats, SH-SY5Y, Ubiquitin Thiolesterase - metabolism, UCH-L1