Details

Autor(en) / Beteiligte

• Liu, Yuyao
• Yan, Jiayin
• Chen, Luxian
• Liao, Yuwei
• Huang, Lijia
• Tan, Jiali

Titel

Multifunctionalized and Dual‐Crosslinked Hydrogel Promotes Inflammation Resolution and Bone Regeneration via NLRP3 Inhibition in Periodontitis

Ist Teil von

• Small structures, 2024-03, Vol.5 (3), p.n/a

Ort / Verlag

Weinheim: Wiley Subscription Services, Inc

Erscheinungsjahr

2024

Link zum Volltext

Quelle

Wiley Online Library - AutoHoldings Journals

Beschreibungen/Notizen

• Alveolar bone resorption caused by bacteria‐induced periodontitis remains challenging due to sustained inflammation. Periodontal pathogens like Porphyromonas gingivalis launch the primed signal of NOD‐like receptor family pyrin domain‐containing 3 (NLRP3) inflammasome in macrophages; consequent overproduction of proinflammatory cytokines and reactive oxygen species (ROS) leads to tissue destruction. This provides potential targets for a new therapeutic strategy. Herein, a multifunctionalized and dual‐crosslinked hydrogel pGM/cPL@NI with NLRP3 inhibitor MCC950 loaded is prepared. Driven by the strategic functionalization of gelatin methacryloyl and ε‐poly‐lysine with phenylboronic acid and catechol, respectively, pGM/cPL@NI containing dynamic and photo‐crosslinking networks demonstrates superior mechanical strength and stimuli‐responsive behavior, as well as the overwhelmed performance in bacteria killing and ROS scavenging. Crucially, pGM/cPL@NI restores the compromised osteogenesis by specifically suppressing the proinflammatory cytokine cascade triggered by NLRP3 inflammasome activation and promoting anti‐inflammatory polarization of macrophages. Collectively, pGM/cPL@NI presents robust potential as an effective “cocktail therapy” by combining antibacterial, antioxidant, inflammation resolution, and tissue regenerative functions. The present study reveals the underlying mechanism of the bacterial‐immune‐regeneration cascade and provides an extended approach for periodontal tissue engineering. The multifunctionalized  hydrogel pGM/cPL@NI with reinforced strength  orchestrates functions of antibacteria, reactive oxygen species scavenging, immunomodulation, and osteogenesis promotion. Importantly, the correlation between Porphyromonas gingivalis‐induced NLRP3 inflammasome activation and periodontitis is highlighted, which is effectively blocked by responsively releasing MCC950 from pGM/cPL@NI. The findings provide a novel strategy for rescuing bacteria‐induced inflammatory bone loss in periodontitis.