Details

Autor(en) / Beteiligte

• Diaz, Giovanni H.
• Heller, Stefan

Titel

Fluorescent in situ mRNA detection in the adult mouse cochlea

Ist Teil von

• STAR protocols, 2021-09, Vol.2 (3), p.100711-100711, Article 100711

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2021

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Ossification and the delicateness of the cochlear duct make histologic assessments of the mature cochlea a challenging endeavor. Treatments to soften the bone facilitate sectioning and dissection of the cochlear duct but limit in situ mRNA detection in such specimens. Here, we provide a protocol for in situ mRNA detection using hybridization chain reaction in whole-mount preparations of the adult mouse cochlea. We show examples for multi-probe detection of different mRNAs and describe combination of this method with conventional immunohistochemistry. [Display omitted] •Instructions for preparing the adult mouse cochlea for whole-mount histology applications•Modifications for hybridization chain reaction protocol•Adult cochlea mRNA in situ detection paired with immunostaining and imaging Ossification and the delicateness of the cochlear duct make histologic assessments of the mature cochlea a challenging endeavor. Treatments to soften the bone facilitate sectioning and dissection of the cochlear duct but limit in situ mRNA detection in such specimens. Here, we provide a protocol for in situ mRNA detection using hybridization chain reaction in whole-mount preparations of the adult mouse cochlea. We show examples for multi-probe detection of different mRNAs and describe combination of this method with conventional immunohistochemistry.