Details

Autor(en) / Beteiligte

• Arizti-Sanz, Jon
• Freije, Catherine A.
• Stanton, Alexandra C.
• Petros, Brittany A.
• Boehm, Chloe K.
• Siddiqui, Sameed
• Shaw, Bennett M.
• Adams, Gordon
• Kosoko-Thoroddsen, Tinna-Solveig F.
• Kemball, Molly E.
• Uwanibe, Jessica N.
• Ajogbasile, Fehintola V.
• Eromon, Philomena E.
• Gross, Robin
• Wronka, Loni
• Caviness, Katie
• Hensley, Lisa E.
• Bergman, Nicholas H.
• MacInnis, Bronwyn L.
• Happi, Christian T.
• Lemieux, Jacob E.
• Sabeti, Pardis C.
• Myhrvold, Cameron

Titel

Streamlined inactivation, amplification, and Cas13-based detection of SARS-CoV-2

Ist Teil von

• Nature communications, 2020-11, Vol.11 (1), p.5921-5921, Article 5921

Ort / Verlag

London: Nature Publishing Group UK

Erscheinungsjahr

2020

Quelle

MEDLINE

Beschreibungen/Notizen

• The COVID-19 pandemic has highlighted that new diagnostic technologies are essential for controlling disease transmission. Here, we develop SHINE (Streamlined Highlighting of Infections to Navigate Epidemics), a sensitive and specific diagnostic tool that can detect SARS-CoV-2 RNA from unextracted samples. We identify the optimal conditions to allow RPA-based amplification and Cas13-based detection to occur in a single step, simplifying assay preparation and reducing run-time. We improve HUDSON to rapidly inactivate viruses in nasopharyngeal swabs and saliva in 10 min. SHINE’s results can be visualized with an in-tube fluorescent readout — reducing contamination risk as amplification reaction tubes remain sealed — and interpreted by a companion smartphone application. We validate SHINE on 50 nasopharyngeal patient samples, demonstrating 90% sensitivity and 100% specificity compared to RT-qPCR with a sample-to-answer time of 50 min. SHINE has the potential to be used outside of hospitals and clinical laboratories, greatly enhancing diagnostic capabilities. The COVID-19 pandemic has highlighted the need for user-friendly diagnostic techniques. Here, the authors present SHINE, a streamlined and optimised Cas13-based method with accompanying smartphone app for visual diagnosis.