Details

Autor(en) / Beteiligte

• Salzer, Marion Claudia
• Lafzi, Atefeh
• Berenguer-Llergo, Antoni
• Youssif, Catrin
• Castellanos, Andrés
• Solanas, Guiomar
• Peixoto, Francisca Oliveira
• Stephan-Otto Attolini, Camille
• Prats, Neus
• Aguilera, Mònica
• Martín-Caballero, Juan
• Heyn, Holger
• Benitah, Salvador Aznar

Titel

Identity Noise and Adipogenic Traits Characterize Dermal Fibroblast Aging

Ist Teil von

• Cell, 2018-11, Vol.175 (6), p.1575-1590.e22

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2018

Quelle

MEDLINE

Beschreibungen/Notizen

• During aging, stromal functions are thought to be impaired, but little is known whether this stems from changes of fibroblasts. Using population- and single-cell transcriptomics, as well as long-term lineage tracing, we studied whether murine dermal fibroblasts are altered during physiological aging under different dietary regimes that affect longevity. We show that the identity of old fibroblasts becomes undefined, with the fibroblast states present in young skin no longer clearly demarcated. In addition, old fibroblasts not only reduce the expression of genes involved in the formation of the extracellular matrix, but also gain adipogenic traits, paradoxically becoming more similar to neonatal pro-adipogenic fibroblasts. These alterations are sensitive to systemic metabolic changes: long-term caloric restriction reversibly prevents them, whereas a high-fat diet potentiates them. Our results therefore highlight loss of cell identity and the acquisition of adipogenic traits as a mechanism underlying cellular aging, which is influenced by systemic metabolism. [Display omitted] •The identity of old dermal fibroblasts becomes undefined and noisy•Old dermal fibroblasts acquire adipogenic traits•CR and HFD prevent and potentiate fibroblast aging, respectively•Loss of cell identity is a possible mechanism underlying aging Single-cell transcriptomics and long-term lineage tracing outline aging-induced molecular identity changes of skin fibroblasts that lead them to acquire an adipogenic profile sensitive to whole-body metabolic changes.