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The isothermal transformation of wustite (FeO) in the range of 650~350°C was studied using Gleeble 3500 thermal simulated test machine, electron back-scatter diffraction (EBSD) and scanning electron microscopy (SEM). A wustite layer was initially formed by oxidizing low carbon micro-alloyed steel in air at 880°C for 20 seconds. The layer was then isothermally annealed at 650-350°C in argon for up to 10000 seconds. The transformation of FeO was comprised by a proeutectoid reaction and a eutectoid reaction. FeO initially transformed to Fe3O4 by proeutecioid reaction and then the retained FeO transformed to lamellar mixture Fe+Fe3O4 by eutectoid reaction. The proeutectoid and eutectoid reactions both followed a C-curve trend and the nose temperatures were 475°C and 425°C, respectively. An isothermal transformation (IT) diagram was summarized based on our results.