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In order to improve the accuracy of the measurement of battery internal resistance, a lead-acid battery internal resistance measurement system was designed using the AC injection method and LabVIEW software. The system is based on LabVIEW software, design a stable sin signal produce program and data acquisition program. At the same time, Signal conditioning modules which were developed by NI were used to suppress the noise. The experiment results show that the system easy to use and has a high accuracy.