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The landfill leachate is a kind of complicated waste water which is hard to be treated at present. In this paper seven dominant stains that can effectively degrade COD in leachate were isolated by increasing the concentration of leachate. The influence of treated time, temperature, pH on treatment efficiency of leachate with these dominant stains were studied. The results showed that five bacterial strains, one Actinomyces strain and one mould strain possess fairly high rate to eliminate COD in leachate. The optimum time of degradation of COD in leachate was 3 days, and the pH was suitable for strains which the best was 7. In a broad temperature range from 25°C to 35°C, the COD in leachate could be effectively degraded by all of seven strains.