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Power transformer on-line monitoring on dissolved gas in oil is one of the effective and technical means to achieve the transformer state detection and fault diagnosis, and oil-gas permeability is one of the core technologies to implement transformer on-line monitoring. The traditional methods of oil-gas separation such as vacuum method and mechanical oscillation method were unable to satisfy the requirements of transformer on-line monitoring; and the methods which were used commonly in recent years, like dynamic headspace separation, corrugated tube, carrier gas elution etc, have a high rate of gas-separation and have already been used in some on-line monitoring products. However, the problems still exist: easy formation of oil pollution, so the oil can not be recycled and the device structure is relatively complex. This paper based on the separation principle of polymer membrane, proposes mixed hollow fiber membrane made by polytetrafluoroethylene (PTFE) and polyhexafluoropropylene (PHFP), and designs an oil-gas separation test platform formed by the storage tank, oil-gas permeability tank, temperature controller and gas chromatographic analyzer etc, does laboratory research on the oil-gas permeability of the mixed hollow fiber membrane at different temperatures. The results show that, the permeability of the mixed hollow fiber membrane is obviously better than the commonly used single fluoride film or rubber film, seven fault gases H2, CO, CO2, CH4, C2H6, C2H4, and C2H2 can be separated efficiently form transformer oil within 24 hours. More to the point, the equilibrium time is short, the gas permeability is high and the test platform structure is simple, all of these advantages provide a strong guarantee for the development of on-line monitoring technology on dissolved gas in transformer oil.