Details

Autor(en) / Beteiligte

• ARIKAN, Hüseyin
• GÖÇMEN, Bayram
• İĞCİ, Naşit
• AKMAN, Bahadır

Titel

Age-dependent variations in the venom proteins of Vipera kaznakovi Nikolsky, 1909 and Vipera ammodytes (Linnaeus, 1758) (Ophidia: Viperidae)

Ist Teil von

• Turkish journal of zoology, 2014-01, Vol.38, p.216-221

Ort / Verlag

TUBITAK

Erscheinungsjahr

2014

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• Polyacrylamide disc gel electrophoresis and densitometry analysis were used to analyze venom extracts of Vipera kaznakovi (16.5, 30, and 55 cm) and Vipera ammodytes (28.5, 36.7, and 51.5 cm) specimens of 3 different lengths. V. kaznakovi specimens were collected from Hopa (Artvin Province); 2 V. ammodytes specimens were from Ağva (İstanbul Province) and the longest one was from Yuvacık (Kocaeli Province). The electropherograms of the venom protein samples showed age-dependent qualitative and quantitative variations. by i and Atatür (1979) as previously described in detail (Arıkan et al., 2006). Briefly, venom samples were loaded onto the polyacrylamide gels and separated using a Canalco model 1200 electrophoresis apparatus. Gels were stained with 0.5% Amido black to visualize the protein bands and densitometric curves were obtained using a Gelman ACD-15 model 39430 densitometer at 500 nm.The authors received ethical permission for venom sampling (Ege University Animal Experiments Ethics Committee, 2010-43) and special permission for field studies (from Republic of Turkey Ministry of Forestry and Water Affairs).3. ResultsThe venom extracts of Vipera kaznakovi and Vipera ammodytes specimens had a viscosity higher than that of water. Although venom secretions of 2 specimens of V. kaznakovi (16.5 and 30 cm total lengths) were colorless, they were light yellow in the 55-cm-long specimen. In contrast, the venom extracts of 3 specimens of V. ammodytes were light yellow.The gel photographs of the venom protein samples of V. kaznakovi specimens are presented in Figure 1. According to our results, the venom proteins of V. kaznakovi were separated into 12 fractions or fraction groups in the 2 shorter individuals (16.5 and 30 cm), while there were 13 fractions or fraction groups in the gel of the 55-cm-long individual (the longest one). Our comparative results of electrophoretic density curves (protein fractions) revealed significant quantitative differences especially between the 2 shorter individuals, while similar electrophoretic mobility patterns and fraction numbers were observed (Figures 1–4). Additionally, the densities of the venom proteins of the shortest individual differed strongly from the others (Figure 1).A B C Figure 1. Polyacrylamide gel electrophoresis of venoms of V. kaznakovi of different lengths. A. 16.5 cm, B. 30 cm, C. 55 cm (S: Start, junction between the stacking and separation gels).0.225 0.200 0.175 0.150 0.125 0.100 0.075 0.050 0.025 O.D. + s Figure 2. Gel photograph showing the electrophoretic separation of the venom protein sample obtained from the 16.5-cm-long Vipera kaznakovi specimen, together with its densitometric tracing curve (O.D.: Optical density, S: Start, junction between the stacking and separation gels).1.8 1.6 1.4 1.2 1.0 0.8 0.6 0.4 0.2 O.D. + s Figure 3. Gel photograph showing the electrophoretic separation of the venom protein sample obtained from the 30-cm-long Vipera kaznakovi specimen, together with its densitometric tracing curve. For further explanation, see caption of Figure 2.The gel photographs of the venom protein samples of V. ammodytes are presented in Figure 5. Similar results were also found in V. ammodytes. According to our results of the electrophoretic and densitometric analyses, the venom proteins of the 28.5-, 36.7-, and 51.5-cm-long V. ammodytes individuals were separated into 13, 14, and 13 fractions or fraction groups, respectively. Our comparative results of electrophoretic protein fractions and den ...