Details

Autor(en) / Beteiligte

• Provost, Philip J.
• Wolanski, Bohdan S.
• Miller, William J.
• Ittensohn, Oswald L.
• McAleer, William J.
• Hilleman, Maurice R.

Titel

Physical, Chemical and Morphologic Dimensions of Human Hepatitis A Virus Strain CR326

Ist Teil von

• Experimental biology and medicine (Maywood, N.J.), 1975-02, Vol.148 (2), p.532-539

Ort / Verlag

London, England: SAGE Publications

Erscheinungsjahr

1975

Quelle

MEDLINE

Beschreibungen/Notizen

• Summary CR326 human hepatitis A virus purified by isopycnic banding from infected marmoset sera was shown to consist of 27 nm spherical particles on electron microscopic examination. The particles were identified as hepatitis A virus by tests for infectivity and by specific neutralization of infectivity with convalescent human hepatitis A serum. Also, identical 27 nm viruses in liver extracts gave specific reactions with hepatitis A antisera when tested by immune electron microscopy. The buoyant density of the virus in CsCl was 1.34 and it was heat (60°), ether and acid stable but was destroyed by heat (100°), formalin (1:4000) and ultraviolet irradiation. Electron microscopic studies of sections of infected marmoset liver showed intracytoplasmic virus particles, usually in vesicles. Presumptive findings for RNA, together with the intracytoplasmic location of the virus, indicated the virus to be of RNA-type. The attributes of the virus indicate it is closely related to the entero-virus family and not to hepatitis B virus. The authors are grateful to F. Banker, P. Giesa, L. Hoover, W. Fisher, M. Johnston, C. Dennis and R. Roehm for excellent technical assistance. Drs. A. Tytell and A. Klink gave important technical advice.