Molecular membrane biology, 1997, Vol.14 (3), p.125-132
1997
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Details
- Autor(en) / Beteiligte
- Titel
- Phosphatidylserine exposure and aminophospholipid translocase activity in Rh-deficient erythrocytes
- Ist Teil von
- Molecular membrane biology, 1997, Vol.14 (3), p.125-132
- Ort / Verlag
- England: Informa UK Ltd
- Erscheinungsjahr
- 1997
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- Endogenous phosphatidylserine (PS) exposure and lipid transport activity have been investigated for seven unrelated cases of Rhnull erythrocytes. Endogenous PS exposure was measured by prothrombinase activity. Out of six cases studied, two Rhnull samples exhibited abnormal aminophospholipid exposure, as suggested by the measurement of a lower Km of factor Xa for prothrombin. Aminophospholipid translocase activity was measured through the transbilayer redistribution of spin-labelled analogues of phospholipids. Provided that incubation conditions allow the maintainance of intracellular ATP level, no difference was observed between Rhnull and control erythrocytes, clearly indicating that the aminophospholipid translocase and Rh polypeptides are different molecular species.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0968-7688eISSN: 1464-5203DOI: 10.3109/09687689709048172Titel-ID: cdi_crossref_primary_10_3109_09687689709048172
- Format
- –
- Schlagworte
- Adenosine Triphosphate - blood, Biological Transport, Active, Carrier Proteins - blood, Erythrocyte Membrane - metabolism, erythrocytes, Erythrocytes, Abnormal - metabolism, Humans, Lipid Bilayers - metabolism, Membrane Proteins - blood, null, Phosphatidylserines - blood, phospholipid asymmetry, Phospholipid Transfer Proteins, Rh-Hr Blood-Group System - blood, Spin Labels, Thromboplastin - metabolism, translocase