Acta veterinaria Brno, 2010-11, Vol.79 (3), p.431-436
2010
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- Autor(en) / Beteiligte
- Titel
- An assay in microtitre plates for absolute abundance of chicken interferon alpha transcripts
- Ist Teil von
- Acta veterinaria Brno, 2010-11, Vol.79 (3), p.431-436
- Erscheinungsjahr
- 2010
- Quelle
- EZB Free E-Journals
- Beschreibungen/Notizen
- Immunosuppression of commercial chickens is a serious animal health and economic problem. The major causes of the immunosuppression are viruses that suppress transcription of interferon genes, especially interferon alpha. There is a need for monitoring immunosuppression in commercially bred chickens. For this purpose, the absolute abundance of interferon alpha transcripts can be measured in blood of chickens by a suitable assay. Such an assay was used to estimate abundance of chicken interferon alpha in a sample of splenic cells induced with polyinosinic polycytidylic acid. The abundance measured was 29+/-2 attomoles/microg total RNA. This assay can be performed in microtitre plates using samples collected from chickens in poultry houses.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0001-7213eISSN: 1801-7576DOI: 10.2754/avb201079030431Titel-ID: cdi_crossref_primary_10_2754_avb201079030431
- Format
- –
- Schlagworte
- ABSORBANCE, ABSORBANCIA, ARN MENSAJERO, ARN MESSAGER, BROILER CHICKENS, http://www.fao.org/aos/agrovoc#c_26861, http://www.fao.org/aos/agrovoc#c_33924, http://www.fao.org/aos/agrovoc#c_35126, http://www.fao.org/aos/agrovoc#c_3914, http://www.fao.org/aos/agrovoc#c_4668, http://www.fao.org/aos/agrovoc#c_4765, http://www.fao.org/aos/agrovoc#c_4788, http://www.fao.org/aos/agrovoc#c_9435, IMMUNODEPRESSION, IMMUNOSUPPRESSION, INMUNODEPRESION, INTERFERON, INTERFERONAS, INTERFERONS, MEASUREMENT, MEDICION, MESSENGER RNA, MESURE, METHODE, METHODS, METODOS, POLLO DE ENGORDE, POULET DE CHAIR, TRANSCRIPCION, TRANSCRIPTION