Details

Autor(en) / Beteiligte

• Honkawa, Mana
• Kawaji, Isao
• Amano, Sho
• Yamaguchi, Akimobu
• Takeo, Masahiro
• Utsumi, Yuichi

Titel

Vertical Chemical Unit Operation Using Microcapillary Arrays for Immunosorbent Assay

Ist Teil von

• Journal of Photopolymer Science and Technology, 2023/06/15, Vol.36(1), pp.19-23

Ort / Verlag

The Society of Photopolymer Science and Technology(SPST)

Erscheinungsjahr

2023

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• In order to detect substances using microfluidic devices, fluidic filters with microcapillary arrays with a high aspect ratio were fabricated using deep X-ray lithography. Also, the pressure required to move the solution held on the filter to the lower reservoir was 1～10 kPa in the 10～60 µm capillary diameter range. The microcapillary arrays enable vertical fluid control by integrating various unit chemical operations such as valves, reservoirs, mixers, and reaction fields, where the capillary wall surface was used as a reaction field for highly sensitive detection of the nonylphenol. As a result, it was possible to detect concentrations near the natural environmental existence values defined by The Ministry of the Environment, Japan. This would allow the device to be used for the detection of environmental substances. We also detected mouse IgG using a fluidic filter and found the possibility of detecting enzymatic reactions with a trace amount of reagent.