The Journal of parasitology, 1978-08, Vol.64 (4), p.645-650
1978
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Details
- Autor(en) / Beteiligte
- Titel
- Isolation and partial characterization of trehalase from Ascaris muscle
- Ist Teil von
- The Journal of parasitology, 1978-08, Vol.64 (4), p.645-650
- Ort / Verlag
- United States: American Society of Parasitologists
- Erscheinungsjahr
- 1978
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- The tissues of female Ascaris suum were assayed for α , α′-glucoside 1-D-glucohydrolase (trehalase) activity. A soluble form of the enzyme was isolated from muscle tissue and purified approximately 37-fold. The enzyme was specific for trehalose as substrate. The pH optimum for enzymatic activity was found to be 6.0, and the apparent Km for trehalose was estimated to be 2.1 × 10-4 M. The product of the reaction was identified as D-glucose by chemical, chromatographic and enzymatic methods.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0022-3395eISSN: 1937-2345DOI: 10.2307/3279952Titel-ID: cdi_crossref_primary_10_2307_3279952
- Format
- –
- Schlagworte
- Ascaris - enzymology, Chromatography, DEAE-Cellulose, Chromatography, Gel, Disaccharides, Disaccharides - isolation & purification, Enzymes, Humans, Hydrogen-Ion Concentration, Muscles, Muscles - enzymology, Paper chromatography, Parasitism, Parasitology, Quaternary ammonium compounds, Solvents, Substrate Specificity, Sugars, Sulfates, swine, Trehalose - isolation & purification