Details

Autor(en) / Beteiligte

• Kajiwara, Hideyuki
• Murakami, Ritsuko
• Nakajima, Kenichi

Titel

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry-based biotyping of silk. I. Method development

Ist Teil von

• Journal of Electrophoresis, 2021, Vol.65(1), pp.39-47

Ort / Verlag

Japanese Electrophoresis Society

Erscheinungsjahr

2021

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• A method for the analysis of silkworm (Bombyx mori L.) cocoons and silk fibers was established using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. This method was evaluated on the most popular four-way cross hybrid race Kinshu × Showa and its parent races Kinshu and Showa. Most of the peaks observed in the peptide mass fingerprints of the Kinshu × Showa cocoon were from the parent races Kinshu and Showa. Simultaneous acid cleavage of silk fibers at room temperature is a characteristic phenomenon that has not been observed in spider silk and animal hair.