Details

Autor(en) / Beteiligte

• Gerogianni, Irini
• Pitaraki, Eleanna
• Jagirdar, Rajesh M
• Kouliou, Olympia
• Giannakou, Lydia
• Giannopoulos, Stefanos
• Papazoglou, Eleftherios
• Hatzoglou, Chrissi
• Gourgoulianis, Konstantinos I
• Zarogiannis, Sotirios G

Titel

2-Deoxy-glucose Enhances the Effect of Cisplatin and Pemetrexed in Reducing Malignant Pleural Mesothelioma Cell Proliferation But Not Spheroid Growth

Ist Teil von

• Anticancer research, 2019-07, Vol.39 (7), p.3809-3814

Ort / Verlag

Greece

Erscheinungsjahr

2019

Quelle

MEDLINE

Beschreibungen/Notizen

• Malignant pleural mesothelioma (MPM) is a therapy-resistant neoplasm of the pleura. Standard chemotherapy consists of a combination of cisplatin (CPDD) and pemetrexed (PEM). The aim of this study was to assess whether inhibition of aerobic glycolysis by 2-deoxy-glucose (2DG) would enhance the effects of standard chemotherapy. MeT-5A, M14K, MSTO and ZL34 cell lines were used. Cell viability with 2DG and cell proliferation and spheroid formation with CPDD+PEM alone and with 2-DG were tested. Viability with 2-DG was dose-dependent. Cell proliferation with CPDD+PEM on 2D surface was reduced in all cell types, 2-DG inclusion demonstrated a synergistic effect in MSTO and ZL34 cells. Spheroid growth in 3D with CPDD+PEM or CPDD+PEM+2-DG lowered spheroid growth in all cell types. 2-DG synergizes with CPDD+PEM in lowering MPM cell proliferation in 2D to <20%. In 3D MPM spheroid growth 2-DG synergism with CPDD+PEM treatment is not maintained.