Details

Autor(en) / Beteiligte

• Wojtania, Agnieszka
• Węgrzynowicz-Lesiak, Elżbieta
• Dziurka, Michał
• Waligórski, Piotr

Titel

Sucrose and Cytokinin Interactions in Relation to Ethylene and Abscisic Acid Production in the Regulation of Morphogenesis in Pelargonium × hortorum L.H. Bailey In Vitro

Ist Teil von

• Acta biologica cracoviensia. Series Botanica., 2015-06, Vol.57 (1), p.62-69

Ort / Verlag

De Gruyter Open

Erscheinungsjahr

2015

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• The aim of the study was to determine the effect of exogenous sucrose and cytokinin on ethylene production and responsiveness in relation to the shoot formation of × ‘Bergpalais’ in vitro. Increasing the concentration of sucrose from 15 to 40 g L in medium containing -topolin ( T) resulted in a two-fold decrease in the number of shoots and leaves as well as a reduction in ethylene production. The addition of ethylene synthesis inhibitor (AVG) to T-medium significantly reduced the ethylene production and the shoot growth, but it had no significant influence on the shoot formation. The T-induced shoot formation was, however, significantly reduced in the presence of ethylene action inhibitor (AgNO ), in a manner dependent on sucrose levels. At the end of the subculture period, increased sucrose concentrations (15–40 g L ) in the presence of T and AgNO resulted in a 3.7-fold increase in ethylene emission. At the same time, the supply of sucrose caused a 2.8-fold increase in the level of endogenous abscisic acid (ABA). Our results may suggest that the inhibitory effect of high sucrose concentration (30 and 40 g L ) may depend on its influence on ethylene sensitivity. It also suggests that sucrose-regulation of the shoot formation of in vitro is mediated by ABA.