Details

Autor(en) / Beteiligte

• Wei, Ming-Tzo
• Yang, Kun-Ta
• Karmenyan, Artahses
• Chiou, Arthur

Titel

Three-dimensional optical force field on a Chinese hamster ovary cell in a fiber-optical dual-beam trap

Ist Teil von

• Optics express, 2006-04, Vol.14 (7), p.3056

Ort / Verlag

United States

Erscheinungsjahr

2006

Quelle

EZB-FREE-00999 freely available EZB journals

Beschreibungen/Notizen

• We used a fiber-optical dual-beam trap (single-mode fiber, lambda = 532nm, trapping power ~ 22mW, the distance between the two fiber end-faces = 125mum) to capture a Chinese hamster ovary (CHO) cell with a diameter of approximately 15mum and tracked its three-dimensional Brownian motion via a pair of orthogonal quadrant photodiodes. By analyzing the Brownian motion of the trapped CHO cell, we determined the force constants of the optical force field on the CHO cell to be k(x)=6.75 pN/mum, k(y)=5.53 pN/mum, k(z)=1.96 pN/mum, and k(x)=2.91 pN/mum, k(y)=2.7 pN/mum, k(z)=0.79 pN/mum, respectively, before and after the CHO cell was treated with latrunculin, a toxic drug known to disrupt the cytoskeleton of the cell.