Details

Autor(en) / Beteiligte

• Twyford, Alex D.
• Beasley, Jordan
• Barnes, Ian
• Allen, Heather
• Azzopardi, Freja
• Bell, David
• Blaxter, Mark L.
• Broad, Gavin
• Campos-Dominguez, Lucia
• Choonea, Darren
• Crowley, Liam
• Cuber, Piotr
• Cunliffe, Michael
• Dombrowski, Alexandra
• Douglas, Brian
• Forrest, Laura L.
• Gaya, Ester
• Greeves, Clementine
• Griffin, Claire
• Harley, Joanna
• Hart, Michelle L.
• Holland, Peter W.H.
• Hollingsworth, Peter M.
• Januszczak, Inez
• Jones, Amanda
• Kersey, Paul
• Kilias, Estelle
• Lawniczak, Mara K.N.
• Lewis, Owen T.
• Mian, Sahr
• Minotto, Alice
• Misra, Raju
• Mulhair, Peter O.
• Pereira da Conceicoa, Lyndall
• Price, Ben W.
• Salatino, Silvia
• Shaw, Felix
• Sivell, Olga
• Sivess, Laura
• Uhl, Rebekka
• Woof, Kieran

Titel

A DNA barcoding framework for taxonomic verification in the Darwin Tree of Life Project

Ist Teil von

• Wellcome open research, 2024, Vol.9, p.339

Erscheinungsjahr

2024

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Biodiversity genomics research requires reliable organismal identification, which can be difficult based on morphology alone. DNA-based identification using DNA barcoding can provide confirmation of species identity and resolve taxonomic issues but is rarely used in studies generating reference genomes. Here, we describe the development and implementation of DNA barcoding for the Darwin Tree of Life Project (DToL), which aims to sequence and assemble high quality reference genomes for all eukaryotic species in Britain and Ireland. We present a standardised framework for DNA barcode sequencing and data interpretation that is then adapted for diverse organismal groups. DNA barcoding data from over 12,000 DToL specimens has identified up to 20% of samples requiring additional verification, with 2% of seed plants and 3.5% of animal specimens subsequently having their names changed. We also make recommendations for future developments using new sequencing approaches and streamlined bioinformatic approaches.