Details

Autor(en) / Beteiligte

• Chamoux, Estelle
• Narcy, Agnàs
• Lehoux, Jean-Guy
• Gallo-Payet, Nicole

Titel

Fibronectin, Laminin, and Collagen IV as Modulators of Cell Behavior during Adrenal Gland Development in the Human Fetus

Ist Teil von

• The journal of clinical endocrinology and metabolism, 2002-04, Vol.87 (4), p.1819-1828

Ort / Verlag

Bethesda, MD: Endocrine Society

Erscheinungsjahr

2002

Quelle

Oxford Journals 2020 Medicine

Beschreibungen/Notizen

• The specific development of the human fetal adrenal gland requires cell proliferation, migration, apoptosis, and zone-specific steroidogenic activity. The present work was designed to determine the physiological significance of the previously identified spatial distribution of extracellular matrix components in the fetal gland. Primary cultures of human fetal adrenal cells grown on collagen IV, laminin, or fibronectin revealed that cell morphology was affected by environmental cues. Matrices also modulated the profile of steroid secretion by the fetal cells. Collagen IV favored cortisol secretion after ACTH or angiotensin II stimulation and increased dehydroepiandrosterone production when the AT2 receptor of angiotensin II was specifically stimulated. These effects were correlated by changes in the mRNA levels of 3β-hydroxysteroid dehydrogenase and cytochrome P450C17. In contrast, fibronectin and laminin decreased cell responsiveness to ACTH in terms of cortisol secretion, but enhanced ACTH-stimulated androgen secretion. Finally, extracellular matrices were able to orchestrate cell behavior. Collagen IV and laminin enhanced cell proliferation, and fibronectin increased cell death. This study is the first to demonstrate that the nature of extracellular matrix coordinates specific steroidogenic pathways and cell turnover in the developing human fetal adrenal gland.