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Fas and Fas Ligand Expression in Fetal and Adult Human Testis with Normal or Deranged Spermatogenesis
Ist Teil von
The journal of clinical endocrinology and metabolism, 2000-08, Vol.85 (8), p.2692-2700
Ort / Verlag
Endocrine Society
Erscheinungsjahr
2000
Link zum Volltext
Quelle
Oxford Journals 2020 Medicine
Beschreibungen/Notizen
In mice, the Fas/Fas ligand (FasL) system has been shown to be involved
in germ cell apoptosis. In the present study we evaluated the
expression of Fas and Fas ligand (FasL) in fetal and adult human
testis. Semiquantitative RT-PCR demonstrated the expression of Fas and
FasL messenger ribonucleic acids in adult testis, but not in fetal
testis (20–22 weeks gestation). In situ RT-PCR and
immunohistochemistry experiments on adult human testis demonstrated the
expression of FasL messenger ribonucleic acid and protein in Sertoli
and Leydig cells, whereas the expression of Fas was confined to the
Leydig cells and sporadic degenerating spermatocytes. The number of
Fas-positive germ cells per 100 Sertoli cell nuclei was increased in 10
biopsies with postmeiotic germ cell arrest compared to 10 normal testis
biopsies (mean, 3.82 ± 0.45 vs. 2.02 ± 0.29;
P = 0.0001), but not in 10 biopsies with meiotic
germ cell arrest (mean, 1.56 ± 1.07). Fas and FasL proteins were
not expressed in cases of idiopathic hypogonadotropic hypogonadism.
Together, these findings may suggest that Fas/FasL expression in the
human testis is developmentally regulated and under gonadotropin
control. The increased germ cell expression of Fas in patients with
postmeiotic germ cell arrest suggests that the Fas/FasL system may be
involved in the quality control mechanism of the produced gametes.