UNIVERSI
TÄ
TS-
BIBLIOTHEK
P
ADERBORN
Anmelden
Menü
Menü
Start
Hilfe
Blog
Weitere Dienste
Neuerwerbungslisten
Fachsystematik Bücher
Erwerbungsvorschlag
Bestellung aus dem Magazin
Fernleihe
Einstellungen
Sprache
Deutsch
Deutsch
Englisch
Farbschema
Hell
Dunkel
Automatisch
Sie befinden Sich nicht im Netzwerk der Universität Paderborn. Der Zugriff auf elektronische Ressourcen ist
gegebenenfalls
nur via VPN oder Shibboleth (DFN-AAI) möglich.
mehr Informationen...
Universitätsbibliothek
Katalog
Suche
Details
Zur Ergebnisliste
Ergebnis 14 von 18
Datensatz exportieren als...
BibTeX
Long-Term Generation of Colony-Forming Cells in Liquid Culture of CD34+ Cord Blood Cells in the Presence of Recombinant Human Stem Cell Factor
Blood, 1992-05, Vol.79 (10), p.2620-2627
Giovanni, Migliaccio
Anna Rita, Migliaccio
Maurice, L. Druzin
Patricia-Jane, V. Giardina
Krisztina, M. Zsebo
John, W. Adamson
1992
Volltextzugriff (PDF)
Details
Autor(en) / Beteiligte
Giovanni, Migliaccio
Anna Rita, Migliaccio
Maurice, L. Druzin
Patricia-Jane, V. Giardina
Krisztina, M. Zsebo
John, W. Adamson
Titel
Long-Term Generation of Colony-Forming Cells in Liquid Culture of CD34+ Cord Blood Cells in the Presence of Recombinant Human Stem Cell Factor
Ist Teil von
Blood, 1992-05, Vol.79 (10), p.2620-2627
Ort / Verlag
Washington, DC: Elsevier Inc
Erscheinungsjahr
1992
Quelle
MEDLINE
Beschreibungen/Notizen
Human cord blood was used as a source of progenitor and stem cells to evaluate the effect of recombinant human stem-cell factor (SCF) on colony formation and the generation of colony-forming cells (CFC) under highly defined, serum-deprived conditions. SCF interacted with a number of hematopoietic growth factors to stimulate colony growth and was particularly effective in stimulating the formation of mixed-cell colonies from CD34+ soybean agglutinin negative (SBA- ) cells. In suspension culture of CD34+, SBA- cells, SCF alone was unable to maintain cell numbers or CFC but, in combination with interleukin-3 (IL-3), increased input numbers of cells by 10-fold and increased CFC of all kinds by nearly 20-fold. This included erythroid burst-forming cells (BFU-E), granulocyte/macrophage (GM) CFC, and mixed-cell CFC. In contrast, CD34- SBA- cells neither gave rise to CFC nor were maintained by combinations of growth factors including SCF. SCF interacted with erythropoietin (Epo) and granulocyte colony-stimulating factor (G-CSF) to maintain large numbers of cells as well as to generate a twofold to threefold increase in CFC in the case of Epo, and a 10-fold increase in CFC in the case of G-CSF. With Epo, the predominant CFC generated were BFU-E and erythroid CFC and many of the cells in suspension were erythroblasts. In contrast, SCF plus G-CSF resulted in large numbers of granulocytes at various stages of maturation and the CFC generated were almost exclusively granulocytic-CFC. IL-1 and IL-6, alone or in combination with SCF, showed little or no ability to increase cell numbers or generate CFC. In summary, SCF interacts with a variety of hematopoietic growth factors to promote colony formation, particularly mixed-cell colony formation, and also, in suspension culture, SCF interacts with IL-3, G-CSF, and Epo to generate large numbers of differentiated cells as well as a variety of CFC for up to 1 month. © 1992 by The American Society of Hematology.
Sprache
Englisch
Identifikatoren
ISSN: 0006-4971
eISSN: 1528-0020
DOI: 10.1182/blood.V79.10.2620.2620
Titel-ID: cdi_crossref_primary_10_1182_blood_V79_10_2620_2620
Format
–
Schlagworte
Antigens, CD - analysis
,
Antigens, CD34
,
Biological and medical sciences
,
Cell differentiation, maturation, development, hematopoiesis
,
Cell Division - drug effects
,
Cell physiology
,
Cells, Cultured
,
Culture Techniques - methods
,
Female
,
Fetal Blood - cytology
,
Fundamental and applied biological sciences. Psychology
,
Granulocyte Colony-Stimulating Factor - pharmacology
,
Granulocyte-Macrophage Colony-Stimulating Factor - pharmacology
,
Hematopoietic Cell Growth Factors - pharmacology
,
Humans
,
Infant, Newborn
,
Interleukin-3 - pharmacology
,
Kinetics
,
Molecular and cellular biology
,
Pregnancy
,
Recombinant Proteins - pharmacology
,
Stem Cell Factor
,
Stem Cells - cytology
,
Stem Cells - drug effects
,
Time Factors
Weiterführende Literatur
Empfehlungen zum selben Thema automatisch vorgeschlagen von
bX