Details

Autor(en) / Beteiligte

• Xu, Xia
• Wang, Yixiong
• Choi, Wonshik
• Godbout, Roseline

Titel

Abstract 2886: The mechanistic effect of DHA on FABP7 associated membrane lipid order & nanodomain distribution in glioblastoma migration

Ist Teil von

• Cancer research (Chicago, Ill.), 2021-07, Vol.81 (13_Supplement), p.2886-2886

Erscheinungsjahr

2021

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• Abstract Glioblastoma (GBM) is the most common primary brain malignancy. Brain fatty acid binding protein (B-FABP, FABP7) promotes glioblastoma (GBM) migration and correlates to dismal prognosis in GBM patients. Long chain polyunsaturated fatty acids (PUFAs) such as docosahexaenoic acid (DHA) and arachidonic acid (AA) are abundant in brain cell membranes. DHA and AA are preferred ligands of FABP7, with DHA having the stronger affinity for FABP7. Previous studies have shown that DHA inhibits GBM migration in an FABP7-dependent manner. In the current study, we have demonstrated that DHA dramatically altered membrane FABP7 nanoscale distribution pattern, which resulting from plasma membrane lipid ordered domain disruption in GBM cells and neurosphere cultures. We used quantitative membrane order assay to show that FABP7-expressing cells has increased membrane lipid order which is tightly correlated with higher GBM migration property. Using stimulated emission depletion (STED) microscopy, we observed lipid ordered domain was associated with FABP7 nanoscale domain formation. Interestingly, super-resolution microscopy quantitative image analysis revealed that FABP7 membrane nanodomains distribution was dramatically altered upon DHA treatment, which is associated with DHA inhibitory effect on membrane lipid order. Furthermore, STED microscopy has also revealed that reduced FABP7 nanodomain formation in slow migrating GBM cells are similar as FABP7-expressing GBM cells upon DHA treatment. Therefore, we hypothesize that these alterations are likely to be the mechanism of FABP7-mediated DHA inhibitory role in GBM migration. Citation Format: Xia Xu, Yixiong Wang, Wonshik Choi, Roseline Godbout. The mechanistic effect of DHA on FABP7 associated membrane lipid order & nanodomain distribution in glioblastoma migration [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2021; 2021 Apr 10-15 and May 17-21. Philadelphia (PA): AACR; Cancer Res 2021;81(13_Suppl):Abstract nr 2886.