Details

Autor(en) / Beteiligte

• Friedrich, Ivar
• Spillner, Jan
• Lu, Er-Xiong
• Barnscheidt, Markus
• Kuss, Oliver
• Sablotzki, Armin
• Schade, F. Ulrich
• Borgermann, Jochen

Titel

Induction of endotoxin tolerance improves lung function after warm ischemia in dogs

Ist Teil von

• American journal of physiology. Lung cellular and molecular physiology, 2003-01, Vol.284 (1), p.224-L231

Ort / Verlag

United States

Erscheinungsjahr

2003

Quelle

MEDLINE

Beschreibungen/Notizen

• 1  Thoracic and Cardiovascular Surgery, 3  Institute of Epidemiology, Biometrics and Informatics, Martin-Luther-University, 06097 Halle; and 2  Clinical Research Group Shock and Multiorgan Failure, Essen University, 45128 Essen, Germany In shock models, induction of endotoxin tolerance (ET) is known to have a protective effect. The present study was designed to explore if ET is effective in protecting lungs from reperfusion injury. Twelve foxhounds were used as experimental animals. After a left thoracotomy, the left hilum was clamped for 3 h, followed by 8 h of reperfusion. In the treatment group (ET, n  = 6), dogs were pretreated with incremental daily endotoxin doses of up to 60 µg/kg on day 6 . The ischemia and reperfusion experiment was carried out on day 9 . Control group animals ( n  = 6) were not subjected to endotoxin. After 8 h of observation, functional parameters of the reperfused lung of the ET and the control group were statistically different ( P  < 0.05) with respect to P O 2 [ET vs. control: 172.7   ± 12.9 vs. 66.1 ± 7.2 (SE) mmHg], compliance (16.0 ± 1.2 vs. 8.3   ± 1.0 ml/0.1 kPa), and the wet-to-dry ratio (9.4 ± 0.8 vs. 16.7   ± 1.2). After 3 h of warm ischemia and 8 h of reperfusion, pulmonary function and lung water content improved in the endotoxin-tolerant group. ischemia-reperfusion; acute respiratory distress syndrome; surfactant; lipopolysaccharide