Details

Autor(en) / Beteiligte

• Kunkel, E. J
• Jung, U
• Ley, K

Titel

TNF-alpha induces selectin-mediated leukocyte rolling in mouse cremaster muscle arterioles

Ist Teil von

• American journal of physiology. Heart and circulatory physiology, 1997-03, Vol.272 (3), p.H1391-H1400

Ort / Verlag

United States

Erscheinungsjahr

1997

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• E. J. Kunkel, U. Jung and K. Ley Department of Biomedical Engineering, University of Virginia School of Medicine, Charlottesville 22908, USA. Leukocyte rolling is commonly restricted to venules and mediated by selectins expressed both on leukocytes (L-selectin) and the vascular endothelium (P- and E-selectin). We show here that 2- to 3-h tumor necrosis factor-alpha (TNF-alpha) stimulation of the mouse cremaster muscle induces rolling in arterioles (diameters 30-70 microm; wall shear rates 225-1,770 s(-1)). Weak P-selectin expression was detected on arteriolar endothelium of TNF-alpha-stimulated cremaster muscles. No rolling was observed in arterioles smaller than 30 microm (wall shear rates 1,500-3,250 s(-1)). The arteriolar rolling flux fraction in wild-type mice averaged approximately 5% and rolling was blocked by the P-selectin monoclonal antibody (MAb) RB40.34. Rolling in L- and E-selectin-deficient mice was similar to that in wild-type mice and was also blocked by the MAb RB40.34. Rolling was completely absent in arterioles of P-selectin-deficient mice. The average rolling velocity in arterioles of wild-type and L-selectin-deficient mice was approximately 50 microm/s but increased to approximately 110 microm/s in E-selectin-deficient mice and after injection of the blocking E-selectin MAb 9A9 in wild-type mice. We conclude that TNF-alpha treatment induces P-selectin-dependent rolling in arterioles that requires E-selectin for rolling at normal velocities.