Details

Autor(en) / Beteiligte

• Kochukov, Mikhail Y
• McNearney, Terry A
• Fu, Yibing
• Westlund, Karin N

Titel

Thermosensitive TRP ion channels mediate cytosolic calcium response in human synoviocytes

Ist Teil von

• American Journal of Physiology: Cell Physiology, 2006-09, Vol.291 (3), p.C424-C432

Ort / Verlag

United States: American Physiological Society

Erscheinungsjahr

2006

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Departments of 1 Neuroscience and Cell Biology, 2 Internal Medicine, and 3 Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas Submitted 28 October 2005 ; accepted in final form 17 March 2006 The transient receptor potential (TRP) channels are important membrane sensors, responding to thermal, chemical, osmotic, or mechanical stimuli by activation of calcium and sodium fluxes. In this study, three distinct TRP channels were detected and their role established in mediating cytosolic free calcium concentration ([Ca 2+ ] cyt ) response in tumor-derived SW982 synoviocytes and primary cultures of human synovial cells from patients with inflammatory arthropathies. As shown by fura-2 ratio measurements while cells were incubated in a temperature-regulated chamber, significant [Ca 2+ ] cyt elevation was elicited by rapid changes in bath temperature, application of TRPV1 receptor agonists capsaicin and resiniferatoxin, or a cold receptor stimulator, icilin. Temperature thresholds for calcium response were determined to be 12 ± 1°C for cold and 28 ± 2°C for heat activation. Temperature increases or decreases beyond these thresholds resulted in a significant rise in the magnitude of [Ca 2+ ] cyt spikes. Observed changes in [Ca 2+ ] cyt were completely abolished in calcium-free medium and thus resulted from direct calcium entry through TRP channels rather then by activation of voltage-dependent calcium channels. Two heat sensitive channels, TRPV1 and TRPV4, and a cold-sensitive channel, TRPA1, were detected by RT-PCR. Minimal mRNA for TRPV3 or TRPM8 was amplified. The RT-PCR results support the data obtained with the [Ca 2+ ] cyt measurements. We propose that the TRP channels are functionally expressed in human synoviocytes and may play a critical role in adaptive or pathological changes in articular surfaces during arthritic inflammation. transient receptor potential channels; vanilloid receptors; arthritis Address for reprint requests and other correspondence: K. N. Westlund High, Dept. of Neuroscience and Cell Biology, Univ. of Texas Medical Branch, Medical Research Bldg., Rm. 10.138D, 301 University Blvd., Galveston, TX 77555-1043 ( kwhigh{at}utmb.edu )