Details

Autor(en) / Beteiligte

• Maerle, A. V.
• Ryazantsev, D. Yu
• Dmitrenko, O. A.
• Petrova, E. E.
• Komaleva, R. L.
• Sergeev, I. V.
• Trofimov, D. Yu
• Zavriev, S. K.

Titel

Detection of Staphylococcus aureus toxins using immuno-PCR

Ist Teil von

• Russian journal of bioorganic chemistry, 2014-09, Vol.40 (5), p.526-531

Ort / Verlag

Moscow: Pleiades Publishing

Erscheinungsjahr

2014

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• A highly sensitive test system, based on the immuno-PCR method, was developed for the detection of two staphylococcal toxins: enterotoxin A (SEA) and toxic shock syndrome toxin (TSST). A key element of the developed systems was to obtain supramolecular complexes of bisbiotinylated oligodeoxynucleotides and streptavidin, which were to be used as DNA-tags. Specificity studies showed no cross-reactivity when determining SEA and TSST. The sensitivity of detection of these toxins in the culture supernatants S. aureus was not lower than 10 pg/mL.