Sie befinden Sich nicht im Netzwerk der Universität Paderborn. Der Zugriff auf elektronische Ressourcen ist gegebenenfalls nur via VPN oder Shibboleth (DFN-AAI) möglich. mehr Informationen...
MiR-222-3p Regulates the Proliferation and Differentiation of C2C12 Myoblasts by Targeting BTG2
Ist Teil von
Molecular biology (New York), 2019, Vol.53 (1), p.38-44
Ort / Verlag
Moscow: Pleiades Publishing
Erscheinungsjahr
2019
Link zum Volltext
Quelle
SpringerLink
Beschreibungen/Notizen
MiR-222-3p has been implicated in tumor cell proliferation and has an important role in the differentiation and maturation of myogenic cells. However, its role in skeletal myoblast proliferation is still unclear. In this study, we found that miR-222-3p expression increases initially and then decreases during C2C12 myoblast proliferation. Using synthetic miRNA mimics and inhibitors in gain- or loss-of-function experiments, we showed that miR-222-3p overexpression in C2C12 cells promotes myoblast proliferation and represses myofiber formation, while miR-222-3p downregulation has the opposite effect. Using a prediction program,
BTG2
was identified as a possible target gene of miR-222-3p. During myogenesis, miR-222-3p mimics repress
BTG2
expression, while miR-222-3p inhibitors promote
BTG2
expression. Using dual-luciferase reporter assay, we further demonstrated that miR-222-3p specifically targets
BTG2
. Additionally, we show that siRNA-mediated downregulation of
BTG2
expression in C2C12 myoblasts promotes the proliferation and suppresses differentiation. In conclusion, we provide a novel insight into the mechanism by which miR-222-3p regulates the proliferation and differentiation of C2C12 myoblasts by targeting
BTG2
. This information contributes to our understanding of the role of miRNAs in skeletal muscle development.