Details

Autor(en) / Beteiligte

• Bouffartigues, Emeline
• Gicquel, Gwendoline
• Bazire, Alexis
• Bains, Manjeet
• Maillot, Olivier
• Vieillard, Julien
• Feuilloley, Marc G. J
• Orange, Nicole
• Hancock, R. E. W
• Dufour, Alain
• Chevalier, Sylvie

Titel

Transcription of the oprF Gene of Pseudomonas aeruginosa Is Dependent Mainly on the SigX Sigma Factor and Is Sucrose Induced

Ist Teil von

• Journal of Bacteriology, 2012-08, Vol.194 (16), p.4301-4311

Ort / Verlag

Washington, DC: American Society for Microbiology

Erscheinungsjahr

2012

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• The OprF porin is the major outer membrane protein of Pseudomonas aeruginosa. OprF is involved in several crucial functions, including cell structure, outer membrane permeability, environmental sensing, and virulence. The oprF gene is preceded by the sigX gene, which encodes the poorly studied extracytoplasmic function (ECF) sigma factor SigX. Three oprF promoters were previously identified. Two intertwined promoters dependent on σ70 and SigX are located in the sigX-oprF intergenic region, whereas a promoter dependent on the ECF AlgU lies within the sigX gene. An additional promoter was found in the cmpX-sigX intergenic region. In this study, we dissected the contribution of each promoter region and of each sigma factor to oprF transcription using transcriptional fusions. In Luria-Bertani (LB) medium, the oprF-proximal region (sigX-oprF intergenic region) accounted for about 80% of the oprF transcription, whereas the AlgU-dependent promoter had marginal activity. Using the sigX mutant PAOSX, we observed that the SigX-dependent promoter was largely predominant over the σ70-dependent promoter. oprF transcription was increased in response to low NaCl or high sucrose concentrations, and this induced transcription was strongly impaired in the absence of SigX. The lack of OprF itself increased oprF transcription. Since these conditions led to cell wall alterations, oprF transcription could be activated by signals triggered by perturbation of the cell envelope.