Details

Autor(en) / Beteiligte

• Puga Yung, Gisella
• Bongoni, Anjan K.
• Pradier, Amandine
• Madelon, Natacha
• Papaserafeim, Maria
• Sfriso, Riccardo
• Ayares, David L.
• Wolf, Eckhard
• Klymiuk, Nikolai
• Bähr, Andrea
• Constantinescu, Mihai A.
• Voegelin, Esther
• Kiermeir, David
• Jenni, Hansjörg
• Rieben, Robert
• Seebach, Jörg D.

Titel

Release of pig leukocytes and reduced human NK cell recruitment during ex vivo perfusion of HLA ‐E/human CD 46 double‐transgenic pig limbs with human blood

Ist Teil von

• Xenotransplantation (Københaven), 2018-01, Vol.25 (1)

Erscheinungsjahr

2018

Link zum Volltext

Quelle

Wiley-Blackwell Full Collection

Beschreibungen/Notizen

• Abstract Background In pig‐to‐human xenotransplantation, interactions between human natural killer ( NK ) cells and porcine endothelial cells ( pEC ) are characterized by recruitment and cytotoxicity. Protection from xenogeneic NK cytotoxicity can be achieved in vitro by the expression of the non‐classical human leukocyte antigen‐E ( HLA ‐E) on pEC . Thus, the aim of this study was to analyze NK cell responses to vascularized xenografts using an ex vivo perfusion system of pig limbs with human blood. Methods Six pig forelimbs per group, respectively, stemming from either wild‐type (wt) or HLA ‐E/ hCD 46 double‐transgenic (tg) animals, were perfused ex vivo with heparinized human blood for 12 hours. Blood samples were collected at defined time intervals, cell numbers counted, and peripheral blood mononuclear cells analyzed for phenotype by flow cytometry. Muscle biopsies were analyzed for NK cell infiltration. In vitro NK cytotoxicity assays were performed using pEC derived from wt and tg animals as target cells. Results Ex vivo, a strong reduction in circulating human CD 45 leukocytes was observed after 60 minutes of xenoperfusion in both wt and tg limb groups. NK cell numbers dropped significantly. Within the first 10 minutes, the decrease in NK cells was more significant in the wt limb perfusions as compared to tg limbs. Immunohistology of biopsies taken after 12 hours showed less NK cell tissue infiltration in the tg limbs. In vitro , NK cytotoxicity against hCD 46 single tg pEC and wt pEC was similar, while lysis of double tg HLA ‐E/ hCD 46 pEC was significantly reduced. Finally, circulating cells of pig origin were observed during the ex vivo xenoperfusions. These cells expressed phenotypes mainly of monocytes, B and T lymphocytes, NK cells, as well as some activated endothelial cells. Conclusions Ex vivo perfusion of pig forelimbs using whole human blood represents a powerful tool to study humoral and early cell‐mediated rejection mechanisms of vascularized pig‐to‐human xenotransplantation, although there are several limitations of the model. Here, we show that (i) transgenic expression of HLA ‐E/ hCD 46 in pig limbs provides partial protection from human NK cell‐mediated xeno responses and (ii) the emergence of a pig cell population during xenoperfusions with implications for the immunogenicity of xenografts.