Details

Autor(en) / Beteiligte

• Tran, Ann
• Yan, Matthew T S
• Branch, Donald R
• Blacquiere, Megan
• Pineault, Nicolas
• Pasha, Roya
• Clarke, Gwen

Titel

Severe fetal anemia caused by anti-Jr a : Burst forming unit-erythroid colony formation inhibition assay suggesting possible erythroid suppression mechanism

Ist Teil von

• Transfusion (Philadelphia, Pa.), 2023-04, Vol.63 (4), p.877-882

Ort / Verlag

United States

Erscheinungsjahr

2023

Quelle

MEDLINE

Beschreibungen/Notizen

• The Jr blood group system includes a single, high-prevalence antigen, Jr , encoded by the ABCG2 gene. The impact of anti-Jr in pregnancy is variable, ranging from no clinical effect to severe anemia including some fetal deaths. Case reports have postulated that anti-Jr mediated fetal anemia is poorly hemolytic, suggesting other mechanisms of anemia may be involved. We describe the case of severe anti-Jr mediated fetal anemia. At Canadian Blood Services laboratories, maternal anti-Jr was tested for phagocytic activity via a monocyte monolayer assay (MMA) and erythroid suppression via inhibition of burst forming unit-erythroid (BFU-E) colony formation assays. The New York Blood Center sequenced exons 4 and 7 of the ABCG2 gene. Sequencing of exons 4 and 7 of the ABCG2 gene revealed maternal compound heterozygosity for two nonsense mutations at exon 7 (c.706 C > T and c.784G > T). Fetal sequencing revealed the c.706C > T polymorphism. The MMA showed a borderline phagocytic index (around the cutoff of five for both donor segments tested [5 ± 1 and 7 ± 3]). The BFU-E colony formation inhibition assay suggested a dose-dependent inhibition of BFU-E colony formation with inhibition percentages of 4%, 11%, and 43% at maternal serum concentrations of 2%, 5%, and 10%, respectively. Our findings support the hypothesis that anti-Jr may impair erythropoiesis leading to clinically significant fetal/neonatal anemia. A referral to maternal fetal medicine is recommended if anti-Jr is detected in pregnancy, regardless of the titer.