Details

Autor(en) / Beteiligte

• Lai, Yan
• Cuzick, Alayne
• Lu, Xueqing M.
• Wang, Jianqiang
• Katiyar, Neerja
• Tsuchiya, Tokuji
• Le Roch, Karine
• McDowell, John M.
• Holub, Eric
• Eulgem, Thomas

Titel

The Arabidopsis RRM domain protein EDM 3 mediates race‐specific disease resistance by controlling H3K9me2‐dependent alternative polyadenylation of RPP 7 immune receptor transcripts

Ist Teil von

• The Plant journal : for cell and molecular biology, 2019-02, Vol.97 (4), p.646-660

Erscheinungsjahr

2019

Link zum Volltext

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Summary The NLR ‐receptor RPP 7 mediates race‐specific immunity in Arabidopsis. Previous screens for enhanced downy mildew ( edm ) mutants identified the co‐chaperone SGT 1b ( EDM 1) and the PHD ‐finger protein EDM 2 as critical regulators of RPP 7. Here, we describe a third edm mutant compromised in RPP 7 immunity, edm3 . EDM 3 encodes a nuclear‐localized protein featuring an RNA ‐recognition motif. Like EDM 2, EDM 3 promotes histone H3 lysine 9 dimethylation (H3K9me2) at RPP 7 . Global profiling of H3K9me2 showed EDM 3 to affect this silencing mark at a large set of loci. Importantly, both EDM 3 and EDM 2 co‐associate in vivo with H3K9me2‐marked chromatin and transcripts at a critical proximal polyadenylation site of RPP 7 , where they suppress proximal transcript polyadeylation/termination. Our results highlight the complexity of plant NLR gene regulation, and establish a functional and physical link between a histone mark and NLR ‐transcript processing. Significance Statement We are reporting the identification of the RNA‐binding protein EDM3 as a critical contributor to a race‐specific disease resistance mechanism in Arabidopsis thaliana . Most importantly, we are providing clear evidence for a direct physical link between the heterochromatic histone H3 lysine 9 dimethylation mark and transcript polyadenylation.