Sie befinden Sich nicht im Netzwerk der Universität Paderborn. Der Zugriff auf elektronische Ressourcen ist gegebenenfalls nur via VPN oder Shibboleth (DFN-AAI) möglich. mehr Informationen...
Histone lysine methyltransferases Bna SDG 8.A and Bna SDG 8.C are involved in the floral transition in Brassica napus
Ist Teil von
The Plant journal : for cell and molecular biology, 2018-08, Vol.95 (4), p.672-685
Erscheinungsjahr
2018
Quelle
Alma/SFX Local Collection
Beschreibungen/Notizen
Summary
Although increasing experimental evidence demonstrates that histone methylations play important roles in Arabidopsis plant growth and development, little information is available regarding
Brassica napus
. In this study, we characterized two genes encoding homologues of the Arabidopsis histone 3 lysine 36 (H3K36) methyltransferase
SDG
8, namely,
Bna
SDG
8.A
and
Bna
SDG
8.C
. Although no duplication of
SDG
8 homologous genes had been previously reported to occur during the evolution of any sequenced species, a domain‐duplication was uncovered in
Bna
SDG
8.C
. This duplication led to the identification of a previously unknown
NNH
domain in the
SDG
8 homologues, providing a useful reference for future studies and revealing the finer mechanism of
SDG
8 function. One
NNH
domain is present in Bna
SDG
8.A, while two adjacent
NNH
domains are present in Bna
SDG
8.C. Reverse transcriptase‐quantitative polymerase chain reaction analysis revealed similar patterns but with varied levels of expression of
Bna
SDG
8.A/C
in different plant organs/tissues. To directly investigate their function,
Bna
SDG
8.A
/
C
cDNA
was ectopically expressed to complement the Arabidopsis mutant. We observed that the expression of either
Bna
SDG
8.A
or
Bna
SDG
8.C
could rescue the Arabidopsis
sdg8
mutant to the wild‐type phenotype. Using
RNA
i and
CRISPR
/Cas9‐mediated gene editing, we obtained
Bna
SDG
8.A
/
C
knockdown and knockout mutants with the early flowering phenotype as compared with the control. Further analysis of two types of the mutants revealed that
Bna
SDG
8.A/C
are required for H3K36 m2/3 deposition and prevent the floral transition of
B. napus
by directly enhancing the H3K36 m2/3 levels at the
Bna
FLC
chromatin loci. This observation on the floral transition by epigenetic modification in
B. napus
provides useful information for breeding early‐flowering varieties.
Significance Statement
Few studies on epigenetics in rapeseed have been reported and here we revealed that no duplication of the SDG8 histone lysine methyltransferase gene had occurred during the evolution of
Brassicaceae
, but the newfound NNH domain was duplicated within the BnaSDG8.C. The BnaSDG8.A/C directly affect floral transition in
Brassica napus
primarily by changing the deposition level of H3K36me3 on the
BnaFLC2
chromatin, which will provide a useful theoretical guidance for early‐flowering rapeseed breeding.
Sprache
Englisch
Identifikatoren
ISSN: 0960-7412
eISSN: 1365-313X
DOI: 10.1111/tpj.13978
Titel-ID: cdi_crossref_primary_10_1111_tpj_13978
Format
–
Weiterführende Literatur
Empfehlungen zum selben Thema automatisch vorgeschlagen von bX