Details

Autor(en) / Beteiligte

• Giménez, E.
• Muñoz‐Cobo, B.
• Solano, C.
• Amat, P.
• de la Cámara, R.
• Nieto, J.
• López, J.
• Remigia, M.J.
• Garcia‐Noblejas, A.
• Navarro, D.

Titel

Functional patterns of cytomegalovirus ( CMV ) pp65 and immediate early‐1‐specific CD 8 + T cells that are associated with protection from and control of CMV DNA emia after allogeneic stem cell transplantation

Ist Teil von

• Transplant infectious disease, 2015-06, Vol.17 (3), p.361-370

Erscheinungsjahr

2015

Quelle

Wiley Online Library - AutoHoldings Journals

Beschreibungen/Notizen

• Abstract Background The functional profile of cytomegalovirus ( CMV )‐specific CD 8 + T cells that associate with protection from and control of CMV DNA emia in allogeneic stem cell transplant (allo‐ SCT ) recipients remains incompletely characterized. Methods We enumerated pp65 and immediate early ( IE )‐1‐specific CD 8 + T cells expressing interferon‐gamma, tumor necrosis factor‐alpha, and CD 107a, by flow cytometry in 94 patients at days +30 and +60 after allo‐ SCT . Results Fifty of 94 patients had CMV DNA emia within the first 100 days after transplant. CMV ‐specific CD 8 + T‐cell responses (of any functional type) were more likely to be detected in patients who did not display CMV DNA emia than in those who did ( P  = 0.04). Qualitatively, no major differences in the functional signature of CMV ‐specific CD 8 + T cells were noted between patients who had or did not have CMV DNA emia. Patients displaying levels of polyfunctional CD 8 + T cells at day +30 >0.30 cell/μL had a lower risk of CMV DNA emia (positive predictive value 76%, and negative predictive value 43%). Conclusion The presence of polyfunctional CD 8 + T cells (either expressing CD 107a or not) was associated with lower levels of CMV replication, and higher frequency of self‐resolved episodes. The data reported further clarify the role of polyfunctional CD 8 + T cells in control of CMV DNA emia in allo‐ SCT recipients.