Details

Autor(en) / Beteiligte

• Sun, Sheng‐Kai
• Chen, Yi
• Che, Jing
• Konishi, Noriyuki
• Tang, Zhong
• Miller, Anthony J.
• Ma, Jian Feng
• Zhao, Fang‐Jie

Titel

Decreasing arsenic accumulation in rice by overexpressing Os NIP 1;1 and Os NIP 3;3 through disrupting arsenite radial transport in roots

Ist Teil von

• The New phytologist, 2018-07, Vol.219 (2), p.641-653

Erscheinungsjahr

2018

Quelle

Wiley Online Library Journals Frontfile Complete

Beschreibungen/Notizen

• Summary Rice is a major dietary source of the toxic metalloid arsenic. Reducing arsenic accumulation in rice grain is important for food safety. We generated transgenic rice overexpressing two aquaporin genes, Os NIP 1;1 and Os NIP 3;3 , under the control of a maize ubiquitin promoter or the rice OsLsi1 promoter, and tested the effect on arsenite uptake and translocation. Os NIP 1;1 and Os NIP 3;3 were highly permeable to arsenite in Xenopus oocyte assays. Both transporters were localized at the plasma membrane. Knockout of either gene had little effect on arsenite uptake or translocation. Overexpression of Os NIP 1;1 or Os NIP 3;3 in rice did not affect arsenite uptake but decreased root‐to‐shoot translocation of arsenite and shoot arsenic concentration markedly. The overexpressed Os NIP 1;1 and Os NIP 3;3 proteins were localized in all root cells without polarity. Expression of Os NIP 1;1 driven by the OsLsi1 promoter produced similar effects. When grown in two arsenic‐contaminated paddy soils, overexpressing lines contained significantly lower arsenic concentration in rice grain than the wild‐type without compromising plant growth or the accumulation of essential nutrients. Overexpression of Os NIP 1;1 or Os NIP 3;3 provides a route for arsenite to leak out of the stele, thus restricting arsenite loading into the xylem. This strategy is effective in reducing arsenic accumulation in rice grain.