Details

Autor(en) / Beteiligte

• Strano, Cinzia Patricia
• Bella, Patrizia
• Licciardello, Grazia
• Fiore, Alberto
• Lo Piero, Angela Roberta
• Fogliano, Vincenzo
• Venturi, Vittorio
• Catara, Vittoria

Titel

P seudomonas corrugata crpCDE is part of the cyclic lipopeptide corpeptin biosynthetic gene cluster and is involved in bacterial virulence in tomato and in hypersensitive response in N icotiana benthamiana

Ist Teil von

• Molecular plant pathology, 2015-06, Vol.16 (5), p.495-506

Erscheinungsjahr

2015

Link zum Volltext

Quelle

Wiley Online Library E-Journals

Beschreibungen/Notizen

• Summary P seudomonas corrugata   CFBP 5454 produces two kinds of cyclic lipopeptides ( CLP s), cormycin A and corpeptins, both of which possess surfactant, antimicrobial and phytotoxic activities. In this study, we identified genes coding for a putative non‐ribosomal peptide synthetase and an ABC ‐type transport system involved in corpeptin production. These genes belong to the same transcriptional unit, designated crpCDE . The genetic organization of this locus is highly similar to other P seudomonas   CLP biosynthetic clusters. Matrix‐assisted laser desorption ionization‐time of flight‐mass spectrometry ( MALDI‐TOF ‐ MS ) analysis revealed that transporter and synthetase genomic knock‐out mutants were unable to produce corpeptins, but continued to produce cormycin A . This suggests that CrpCDE is the only system involved in corpeptin production in P . corrugata   CFBP 5454. In addition, phylogenetic analysis revealed that the CrpE ABC transporter clustered with the transporters of CLPs with a long peptide chain. Strains depleted in corpeptin production were significantly less virulent than the wild‐type strain when inoculated in tomato plants and induced only chlorosis when infiltrated into N icotiana benthamiana leaves. Thus, corpeptins are important effectors of P . corrugata interaction with plants. Expression analysis revealed that crpC transcription occurs at high cell density. Two LuxR transcriptional regulators, PcoR and RfiA , have a pivotal role in crpC expression and thus in corpeptin production.