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Summary
Nitrogen metabolite repression (
NMR
) in filamentous fungi is controlled by the
GATA
transcription factors
AreA
and
AreB
. While
AreA
mainly acts as a positive regulator of
NMR
‐sensitive genes, the role of
AreB
is not well understood. We report the characterization of
AreB
and its interplay with
AreA
in the gibberellin‐producing fungus
F
usarium fujikuroi
. The
areB
locus produces three different transcripts that each code for functional proteins fully complementing the
areB
deletion mutant that influence growth and secondary metabolism. However, under nitrogen repression, the
AreB
isoforms differ in subcellular localization indicating distinct functions under these conditions. In addition,
AreA
and two isoforms of
AreB
colocalize in the nucleus under low nitrogen, but their nuclear localization disappears under conditions of high nitrogen. Using a bimolecular fluorescence complementation (
BiFC
) approach we showed for the first time that one of the
AreB
isoforms interacts with
AreA
when starved of nitrogen. Cross‐species complementation revealed that some
AreB
functions are retained between
F
. fujikuroi
and
A
spergillus nidulans
while others have diverged. By comparison to other fungi where
AreB
was postulated to function as a negative counterpart of
AreA
,
AreB
can act as both repressor and activator of transcription in
F
. fujikuroi
.
Sprache
Englisch
Identifikatoren
ISSN: 0950-382X
eISSN: 1365-2958
DOI: 10.1111/mmi.12472
Titel-ID: cdi_crossref_primary_10_1111_mmi_12472
Format
–
Weiterführende Literatur
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