Details

Autor(en) / Beteiligte

• Lin, Jing‐wen
• Meireles, Patrícia
• Prudêncio, Miguel
• Engelmann, Sabine
• Annoura, Takeshi
• Sajid, Mohammed
• Chevalley‐Maurel, Séverine
• Ramesar, Jai
• Nahar, Carolin
• Avramut, Cristina M. C.
• Koster, Abraham J.
• Matuschewski, Kai
• Waters, Andrew P.
• Janse, Chris J.
• Mair, Gunnar R.
• Khan, Shahid M.

Titel

Loss‐of‐function analyses defines vital and redundant functions of the P lasmodium rhomboid protease family

Ist Teil von

• Molecular microbiology, 2013-04, Vol.88 (2), p.318-338

Erscheinungsjahr

2013

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Summary Rhomboid‐like proteases cleave membrane‐anchored proteins within their transmembrane domains. In apicomplexan parasites substrates include molecules that function in parasite motility and host cell invasion. While two P lasmodium rhomboids, ROM 1 and ROM 4, have been examined, the roles of the remaining six rhomboids during the malaria parasite's life cycle are unknown. We present systematic gene deletion analyses of all eight P lasmodium rhomboid‐like proteins as a means to discover stage‐specific phenotypes and potential functions in the rodent malaria model, P . berghei . Four rhomboids ( ROM 4, 6, 7 and 8) are refractory to gene deletion, suggesting an essential role during asexual blood stage development. In contrast ROM 1, 3, 9 and 10 were dispensable for blood stage development and exhibited no, subtle or severe defects in mosquito or liver development. Parasites lacking ROM 9 and ROM 10 showed no major phenotypic defects. Parasites lacking ROM 1 presented a delay in blood stage patency following liver infection, but in contrast to a previous study blood stage parasites had similar growth and virulence characteristics as wild type parasites. Parasites lacking ROM 3 in mosquitoes readily established oocysts but failed to produce sporozoites. ROM 3 is the first apicomplexan rhomboid identified to play a vital role in sporogony.